N,NI--bis(2-chloroethyl)carbamate i-tert-butyl ester ingasetshenziswa njenge-intermediate kumakhemikhali emithi kanye nokwakheka kwezinto eziphilayo. Ekuguqulweni kokwenziwa kwezinto eziphilayo, iqembu le-Boc esakhiweni lingasuswa kalula ukuze kutholakale iqembu le-carboxyl elisebenzayo.

Indlela yokwenziwa ihilela ukushukumisa ngokushesha ukumiswa kwe-bis-(2-chloroethyl)amine hydrochloride (5 g, 0.028 mol) ku-dichloromethane (42 mL) nge-a1Isixazululo samanzi esingu-0% sodium hydroxide (28 mL) ekushiseni kweqhwa lokugeza. Isixazululo se-di-tert-butyl dicarbonate (6.11 g, 0.028 mol) ku-dichloromethane (28 mChemicalbook L) bese kufakwa. Ngemva kokuxutshwa ekamelweni lokushisa amahora angu-18.5, i-dichloromethane (30 mL) ifakwa kancane kancane engxubeni yokusabela. Izigaba ezimbili zibe sezihlukaniswa. Isigaba samanzi sikhishwa nge-dichloromethane (30 mL). Izendlalelo eziphilayo ezihlanganisiwe zomiswa phezu kwe-magnesium sulfate engamanzi, zihlungwe, bese zigxiliswa ukuze kutholakale i-N,N-bis(2-chloroethyl)carbamate tert-butyl.