I-Ethylenediaminetetraacetic acid (EDTA) iyi-ejenti ye-metal chelating. Okubizwa ngokuthi i-chelating kusho ukuthi ingahlangana namanye ama-ion ensimbi futhi yenze ama-ion ensimbi aboshwe angasebenzi.

Ngokusebenzisa lesi sici se-EDTA, kunezinhlobo ezimbili zezinhlelo zokusebenza ekuhlolweni;

Olunye uhlobo uhlelo lokusebenza oluhlehlayo, oluvimbela ukusabela okuthile ngokuphazamisa ukuboshwa kwama-ion ensimbi.

Thatha i-ion: Kwezinye izimo, ukubopha ama-ion ensimbi kubalulekile. Sihlanganisa i-EDTA nala ma-ion ensimbi ukuvimbela lokhu kusabela.

Isibonelo, i-blood coagulation idinga ukubamba iqhaza kwe-calcium ions. Sengeza i-EDTA egazini ukuze i-chelate calcium ions nge-EDTA, ngaleyo ndlela sivimbele ukuhlangana kwegazi. Lona umgomo we-EDTA anticoagulation;

Kwesinye isibonelo, ukulungiswa kwesisombululo se-decalcification ekungcoleni kwe-pathological nakho kusebenzisa umphumela we-chelation we-EDTA. Siyazi ukuthi uma izicubu ezine-calcium ephezulu zingancishisiwe, kuzoba nzima ukusika zibe izicucu eziphelele lapho sisika, ngakho-ke sidinga ukunciphisa ngaphambi kokusika.

Kukhona nohlobo lohlelo oluya phambili olukhuthaza ukusabela okuthile ngokuphazamisa ukuboshwa kwama-ion ensimbi.

Isibonelo, engeza i-EDTA kusixazululo sokugaya i-trypsin. Uma i-EDTA ingengeziwe, i-trypsin izobophezela ku-calcium ne-magnesium endaweni yeseli noma endaweni yesiko, ngaleyo ndlela ivimbele umsebenzi we-trypsin.

Ngalesi sikhathi, engeza i-EDTA encane ukuze uvumele i-EDTA ukuthi ihlangane nalawo ma-calcium amahhala, i-magnesium namanye ama-divalent ion. Ngalesi sikhathi, kwakhiwa isimo esihle sokwenzeka kwe-trypsin.

Into emangalisa kakhulu nge-EDTA ukuncibilika kwayo. Iyancibilika kancane emanzini futhi ibonakala imhlophe emanzini. Ngalesi sikhathi, engeza i-sodium hydroxide ukuze ulungise inani layo le-pH. Njengoba i-pH ikhula futhi iphakama, isisombululo siyacaca futhi sicace. Lapho i-pH isisondele ku-8, iba sobala ngokuphelele. Ayiyesabi i-asidi kodwa i-alkali.

I-EDTA inemisebenzi eminingi emangalisayo. Kuyabonakala ukuthi izinto zemvelo ziyimilingo kakhulu, kodwa asizange sikuqaphele.

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