I-ethylenediaminetetraacetic acid (EDTA) iyi-ejenti yokuchela insimbi. Okubizwa ngokuthi i-chelating kusho ukuthi ingahlangana namanye ama-ion ensimbi bese yenza ama-ion ensimbi aboshwe angasebenzi.

Ukusebenzisa lesi sici se-EDTA, kunezinhlobo ezimbili zezicelo ekuhlolweni;

Uhlobo olulodwa ukusetshenziswa okuphambene, okuvimbela ukusabela okuthile ngokuphazamisa ukubopha kwama-ion ensimbi.

Thatha i-ion: Kwezinye izindlela zokusabela, ukubopha kwama-ion ensimbi kubalulekile. Sihlanganisa i-EDTA nalawa ma-ion ensimbi ukuvimbela lokhu kusabela.

Isibonelo, ukujiya kwegazi kudinga ukuhlanganyela kwama-ion e-calcium. Sifaka i-EDTA egazini ukuze sihlanganise ama-ion e-calcium ne-EDTA, ngaleyo ndlela sivimbele ukujiya kwegazi. Lesi yisimiso se-EDTA sokuvimbela ukujiya kwegazi;

Isibonelo, ukulungiswa kwesisombululo sokususa i-calcium ekudayini kwe-pathological kusebenzisa nomphumela we-chelation we-EDTA. Siyazi ukuthi uma izicubu ezine-calcium ephezulu zingasuswa i-calcium, kuzoba nzima ukusika zibe izicubu eziphelele lapho zisikwa, ngakho-ke sidinga ukususa i-calcium ngaphambi kokusikwa.

Kukhona futhi uhlobo lokusetshenziswa phambili olukhuthaza ukusabela okuthile ngokuphazamisa ukubopha kwama-ion ensimbi.

Isibonelo, engeza i-EDTA esixazululweni sokugaya i-trypsin. Uma i-EDTA ingafakwanga, i-trypsin izobopha i-calcium ne-magnesium ebusweni beseli noma endaweni yokukhulisa, ngaleyo ndlela ivimbele umsebenzi we-trypsin.

Ngalesi sikhathi, engeza i-EDTA encane ukuze uvumele i-EDTA ukuthi ihlangane nalawo ma-ion amahhala e-calcium, i-magnesium kanye namanye ama-ion ahlukene. Ngalesi sikhathi, kwakheka isimo esihle sokuvela kwe-trypsin.

Into emangalisa kakhulu nge-EDTA ukuncibilika kwayo. Incibilika kancane emanzini futhi ibonakala imhlophe emanzini. Ngalesi sikhathi, engeza i-sodium hydroxide ukuze ulungise inani layo le-pH. Njengoba i-pH ikhula kakhulu, ikhambi liba sobala kakhulu. Uma i-pH iseduze no-8, iba sobala ngokuphelele. Ayesabi i-asidi kodwa i-alkali.

I-EDTA inezindlela eziningi ezinhle zokusebenzisa. Kuyabonakala ukuthi izinto zemvelo zinomlingo kakhulu, kodwa asikakuqapheli lokho.

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