1. I-Trihydroxymethyl aminomethane yokuqondisa kokuhlanganiswa kwezimboni

Ngenxa yesakhiwo sayo esilula, i-athomu yekhabhoni emaphakathi iyisakhiwo se-tetrahedral, equkethe ama-hydroxymethyl amathathu kanye neqembu lama-amino, elingakwazi ukwenza okuhlukile esikhundleni nokusabela kwe-REDOX, iwuhlobo lwezinga eliphezulu lokuhlanganisa amakhemikhali izinto zokusetshenziswa. Enye yezindlela zokuhlanganisa ze-TAPS yebhafa yenziwa kusetshenziswa i-Tris ne-1, 3-propanolone otshwaleni. Imvamisa, izinto zokusetshenziswa ze-TRIS ezisetshenziselwa ukuhlanganiswa kwezimboni azidingi ukuhlanzeka okuphezulu kwe-reagent. Ukugcizelelwa kwenani elikhulu, ngakho-ke kubiza kakhulu ukuthola umkhiqizi wempahla eluhlaza onomthamo omkhulu.

2. I-Trimethylol amino methane yokuthola isiqondiso se-biochemical

I-Tris ivamise ukusetshenziswa njengesivimbeli sebhayoloji ukuze kugcinwe i-pH yokusabela kwamakhemikhali ezinto eziphilayo. Ngenxa ye-alkalinity yayo ebuthakathaka, i-Tris ivame ukusetshenziswa ngokuhlangana nama-asidi. Isetshenziselwa kakhulu i-nucleic acid, i-protein dissolution buffer, i-protein imprinting buffer, i-DNA imprinting experiment buffer, i-DNA agarose gel electrophoresis buffer, njll. Kuhlelo lokusabela ngenxa yokubandakanyeka kwama-enzyme, amaprotheni namasosha omzimba ahlukahlukene, ngakho-ke izidingo ze-buffer kuphakeme kakhulu, ngakho-ke ukuthengwa kudinga ukukhetha umenzi ngeminyaka eminingi yocwaningo nentuthuko kanye nesipiliyoni sokukhiqiza, ukuhlanzeka okuphezulu, ukungcola okuncane ukuze kuqinisekiswe ukunemba kokuhlolwa kwamakhemikhali ezinto eziphilayo. Umphumela wokushisa we-Tris buffer, ukumunca kalula i-carbon dioxide emoyeni, ukucushwa kwe-buffer kumele kuvalwe kuvalwe ngokuqinile.

3. I-Trimethylol aminomethane yocwaningo lwezemithi nokuthuthukiswa

I-hydroxymethyl aminomethane enye i-alias, okuyi-trometamol, njengoba ingenayo isisekelo se-sodium amino buffer, emanzini omzimba asabela nge-H2CO3, anciphise i-H2CO3, akhiqize i-HCO32 - futhi ngesikhathi esifanayo, angamunca ama-ion e-hydrogen futhi alungise i-acidemia, umphumela wawo unamandla. , futhi ingakwazi ngokusebenzisa ulwelwesi lwamaseli, evame ukusetshenziswa ku-acute metabolic kanye ne-accidemia yokuphefumula; Njengomuthi we-alkali, osetshenziselwa ukulungisa i-acidosis ngaphandle kokubangela ukwanda kokugcinwa kwe-carbon dioxide. Ngenxa yokuthi isetshenziselwa ukwenza imithi, ukukhiqizwa konke komthombo we-TRIS eluhlaza, umzila wenqubo yokukhiqiza, ukuhlanzeka komkhiqizo, okuqukethwe kwe-ion ukungcola kuyizidingo eziqinile, ukuthengwa kwalolu hlobo lwemikhiqizo kufanele kube okuqinile futhi kuqaphe, kufanele kudlule ukuhlolwa okuqinile.

4. I-Trimethylaminomethane isetshenziswa oketshezini lokugcina amagciwane

Esixazululweni sokulondoloza igciwane, i-Tris ingakwazi kuqala ukugcina inani le-pH lesampula, lisebenze njengesigcinalwazi sebhayoloji, futhi uhla lwebhafa lwe-pH lwe-Tris luyilokhu: 7.0 kuya ku-9.0, ikhono lokulondoloza ukukhipha ukuqhekeka kwesampula ngemva kokukhululwa ukuzinza kwe-nucleic acid, ukugwema ukuwohloka kwe-nucleic acid, kanye nokwenza ngcono ukugxila nokuhlanzeka kwe-nucleic acid, kuqinisekisa ukunemba kwemisebenzi elandelayo njengokuhlaziywa kokuhlolwa kwe-nucleic acid, ngenxa yesikhathi esikhethekile sobhubhane, izidingo zokutholwa kwe-nucleic acid, i-Tris inani lokuthenga lizoba likhulu, ngakho-ke phakamisa ukuthi ukuthengwa kwemikhiqizo enjalo kufanele kuqala kuthengwe inani elincane lomkhiqizo ngaphambi kokuthenga isivivinyo esikhulu esiqinisekisile ukuthi ayikho inkinga, futhi kufanele ibe nobuchwepheshe ngemuva kokuthengisa ukuze unikeze ukwesekwa kochwepheshe.