1. I-Trihydroxymethyl aminomethane yesiqondiso sokwenziwa kwezimboni

Ngenxa yesakhiwo sayo esilula, i-athomu yekhabhoni ephakathi iyisakhiwo se-tetrahedral, equkethe i-hydroxymethyl emithathu kanye neqembu lama-amino, elingenza izinhlobo ezahlukene zokushintsha kanye nokusabela kwe-REDOX, uhlobo lwezinto zokusetshenziswa zamakhemikhali ezisezingeni eliphezulu. Enye yezindlela zokwenziwa kwe-buffer TAPS yenziwe kusetshenziswa i-Tris kanye ne-1, 3-propanolone ku-alcohol. Ngokuvamile, izinto zokusetshenziswa ze-TRIS ezisetshenziselwa ukwenziwa kwezimboni azidingi ubumsulwa obukhulu be-reagent. Ukugcizelelwa kusebuningini obukhulu, ngakho-ke kubiza kakhulu ukuthola umenzi wezinto zokusetshenziswa ezinomthamo omkhulu.

2. I-Trimethylol amino methane yokuthola isiqondiso samakhemikhali

I-Tris ivame ukusetshenziswa njenge-buffer yezinto eziphilayo ukugcina i-pH yokusabela kwamakhemikhali. Ngenxa ye-alkalinity yayo ebuthakathaka, i-Tris ivame ukusetshenziswa kanye nama-asidi. Isetshenziswa kakhulu kwi-nucleic acid, i-protein dissolving buffer, i-protein imprinting buffer, i-DNA imprinting experiment buffer, i-DNA agarose gel electrophoresis buffer, njll. Esistimu yokusabela ngenxa yokubandakanyeka kwama-enzyme, amaprotheni kanye nama-antibodies ahlukahlukene, ngakho-ke izidingo ze-buffer ziphakeme kakhulu, ngakho-ke ukuthenga kudinga ukukhetha umenzi oneminyaka eminingi yocwaningo nentuthuko kanye nokuhlangenwe nakho kokukhiqiza, ubumsulwa obuphezulu, ukungcola okuncane ukuqinisekisa ukunemba kokuhlolwa kwamakhemikhali. Umphumela wokushisa kwe-Tris buffer, kulula ukumunca i-carbon dioxide emoyeni, ukucushwa kwe-buffer kumele kuhlanganiswe kahle.

3. I-Trimethylol aminomethane yocwaningo lwemithi kanye nentuthuko

I-hydroxymethyl aminomethane ezintathu zibizwa nangokuthi i-trometamol, njengoba ingenawo isisekelo se-amino buffer se-sodium, emzimbeni isabela ne-H2CO3, yehlisa i-H2CO3, ikhiqize i-HCO32 - futhi ngasikhathi sinye, ingamunca ama-ion e-hydrogen futhi ilungise i-acidemia, umphumela wayo unamandla, futhi ungangena nge-membrane yeseli, evame ukusetshenziswa kwi-acidemia ebukhali ye-metabolic kanye ne-respiratory; Njengomuthi we-alkaline, osetshenziselwa ukulungisa i-acidosis ngaphandle kokubangela ukwanda kokugcinwa kwe-carbon dioxide. Ngenxa yokuthi isetshenziselwa imithi, ukukhiqizwa okuphelele komthombo wezinto zokusetshenziswa ze-TRIS, indlela yenqubo yokukhiqiza, ubumsulwa bomkhiqizo, okuqukethwe kwe-ion okungcolile kuyizidingo eziqinile, ukuthengwa kwalolu hlobo lwemikhiqizo kumele kube okuqinile futhi kuqaphele, kumele kudlule ukuhlolwa okuqinile.

4. I-Trimethylaminomethane isetshenziswa oketshezini lokugcina amagciwane

Esixazululweni sokulondolozwa kwegciwane, i-Tris ingagcina kuqala inani le-pH lesampula, isebenza njenge-buffer yezinto eziphilayo, kanti ububanzi be-pH buffer ye-Tris buyi: 7.0 kuya ku-9.0, ikhono lokugcina ukukhipha ukuqhekeka kwesampula ngemva kokukhululwa kokuqina kwe-nucleic acid, ukugwema ukuwohloka kwe-nucleic acid, nokuthuthukisa ukuhlushwa kanye nobumsulwa be-nucleic acid, ukuqinisekisa ukunemba kwemisebenzi elandelayo njengokuhlaziywa kokuhlolwa kwe-nucleic acid, ngenxa yesikhathi esikhethekile sobhubhane, izidingo zokutholwa kwe-nucleic acid, inani lokuthenga le-Tris lizoba likhulu, ngakho-ke siphakamisa ukuthi uthenge imikhiqizo enjalo kuqala kufanele uthenge inani elincane lomkhiqizo ngaphambi kokuthenga isivivinyo esikhulu esiqinisekisile ukuthi akukho nkinga, futhi kumele ube nobuchwepheshe ngemuva kokuthengisa ukuze unikeze ukwesekwa kobuchwepheshe bochwepheshe.